Dataset Information

Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii.

ABSTRACT: Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and phosphinothricin resistance cassettes were inserted eGFP and mCherry expression cassettes, respectively. These new vectors were then introduced independently into A. tumefaciens and used to transform M. robertsii either in independent events or in one single co-transformation event using an equimolar mixture of A. tumefaciens cultures. The number of transformants obtained by co-transformation was similar to that obtained by the individual transformation events. This method provides an additional strategy for the simultaneous insertion of multiple genes into M. robertsii.

SUBMITTER: Padilla-Guerrero IE

PROVIDER: S-EPMC5541152 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii.

Padilla-Guerrero Israel Enrique IE Bidochka Michael J MJ

Mycobiology 20170630 2

Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and ph ...[more]

PMID: 28781540

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Dataset Information

Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii.

Publications

<i>Agrobacterium</i>-Mediated Co-transformation of Multiple Genes in <i>Metarhizium robertsii</i>.

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