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Deterministic trapping, encapsulation and retrieval of single-cells.

ABSTRACT: We present a novel method for conducting true single-cell encapsulation at very high efficiency for the manipulation of precious samples. Our unique strategy is based on the sequential capture and original encapsulation of single-cells into a series of hydrodynamic traps. We identified two distinct modes of encapsulation and we established their associated design rules. We improved the trapping scheme to reach a near perfect capture efficiency and make it compatible with the encapsulation process. Finally, we developed the complete device operation that permits highly efficient single-cell encapsulation and droplet retrieval. This platform provides the foundation to a fully integrated multiparameter platform that will impact the analysis of tissues at single-cell resolution.

SUBMITTER: Sauzade M

PROVIDER: S-EPMC5541261 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Deterministic trapping, encapsulation and retrieval of single-cells.

Sauzade M M Brouzes E E

Lab on a chip 20170601 13

We present a novel method for conducting true single-cell encapsulation at very high efficiency for the manipulation of precious samples. Our unique strategy is based on the sequential capture and original encapsulation of single-cells into a series of hydrodynamic traps. We identified two distinct modes of encapsulation and we established their associated design rules. We improved the trapping scheme to reach a near perfect capture efficiency and make it compatible with the encapsulation proces ...[more]

PMID: 28585962

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