Dataset Information

Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases.

ABSTRACT:

Purpose

To evaluate the efficacy of sodium hyaluronate (HA) eye drops for the treatment of diabetic ocular surface diseases in mice.

Methods

Male 6- to 8-week-old C57BL/6 mice underwent induction of type 1 diabetes with intraperitoneal injections of streptozotocin, with normal mice as the control. Topical 0.3% HA, 0.1% HA, 0.4% polyethylene glycol eye drops, and normal saline were administered to diabetic mice with an intact or debrided corneal epithelium. Normal saline was applied in the controls. Corneal epithelial wound healing rate, corneal sensation, nerve fiber density, conjunctival goblet cell number, and MUC-5AC content were measured and compared.

Results

Compared with the controls, topical 0.3% HA use in diabetic mice showed significant improvements in the corneal epithelial wound healing rate (48 hours: 91.5% ± 4.8% vs. 79.8% ± 6.1%; P < 0.05), corneal sensitivity (4.1 ± 0.3 cm vs. 3.5 ± 0.3 cm; P < 0.05), nerve fiber density (12.9% ± 2.3% vs. 6.6% ± 2.4%; P < 0.05), conjunctival goblet cell number (31.0 ± 8.4/100 ?m vs. 19.6 ± 7.1/100 ?m; P < 0.05), and MUC-5AC content (12.5 ± 1.4 ng/mg vs. 7.8 ± 1.5 ng/mg protein; P < 0.05). The beneficial effects of 0.3% HA were better than those of 0.1% HA and 0.4% polyethylene glycol.

Conclusions

Topical 0.3% HA treatment promoted corneal epithelial regeneration, improved corneal sensation, and increased density of corneal nerve fibers and conjunctival goblet cells in mice with diabetic ocular surface diseases.

SUBMITTER: Di G

PROVIDER: S-EPMC5542787 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Publications

Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases.

Di Guohu G Qi Xia X Zhao Xiaowen X Zhang Songmei S Zhou Qingjun Q

Cornea 20170901 9

<h4>Purpose</h4>To evaluate the efficacy of sodium hyaluronate (HA) eye drops for the treatment of diabetic ocular surface diseases in mice.<h4>Methods</h4>Male 6- to 8-week-old C57BL/6 mice underwent induction of type 1 diabetes with intraperitoneal injections of streptozotocin, with normal mice as the control. Topical 0.3% HA, 0.1% HA, 0.4% polyethylene glycol eye drops, and normal saline were administered to diabetic mice with an intact or debrided corneal epithelium. Normal saline was applie ...[more]

PMID: 28644234

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Dataset Information

Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases.

Purpose

Methods

Results

Conclusions

Publications

Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases.

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