Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9.
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ABSTRACT:
SUBMITTER: Adikusuma F
PROVIDER: S-EPMC5542798 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Publications
Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9.
Adikusuma Fatwa F Williams Nicole N Grutzner Frank F Hughes James J Thomas Paul P
Molecular therapy : the journal of the American Society of Gene Therapy 20170617 8
CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes. ...[more]
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