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Rapid de novo assembly of the European eel genome from nanopore sequencing reads.

ABSTRACT: We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860?Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2?Mbp) and structural quality. This combination of affordable nanopore sequencing and light weight assembly promises to make high-quality genomic resources accessible for many non-model plants and animals.

SUBMITTER: Jansen HJ

PROVIDER: S-EPMC5543108 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Rapid de novo assembly of the European eel genome from nanopore sequencing reads.

Jansen Hans J HJ Liem Michael M Jong-Raadsen Susanne A SA Dufour Sylvie S Weltzien Finn-Arne FA Swinkels William W Koelewijn Alex A Palstra Arjan P AP Pelster Bernd B Spaink Herman P HP Thillart Guido E van den GEVD Dirks Ron P RP Henkel Christiaan V CV

Scientific reports 20170803 1

We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860 Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2 Mbp) and structural quality. This combination of affordable nanopore sequen ...[more]

PMID: 28775309

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Author Correction: Rapid de novo assembly of the European eel genome from nanopore sequencing reads.

Project description:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.

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