Germline factor DDX4 functions in blood-derived cancer cell phenotypes.

ABSTRACT: DDX4 (the human ortholog of Drosophila Vasa) is an RNA helicase and is present in the germ lines of all metazoans tested. It was historically thought to be expressed specifically in germline, but with additional organisms studied, it is now clear that in some animals DDX4/Vasa functions outside of the germline, in a variety of somatic cells in the embryo and in the adult. In this report, we document that DDX4 is widely expressed in soma-derived cancer cell lines, including myeloma (IM-9) and leukemia (THP-1) cells. In these cells, the DDX4 protein localized to the mitotic spindle, consistent with findings in other somatic cell functions, and its knockout in IM-9 cells compromised cell proliferation and migration activities, and downregulated several cell cycle/oncogene factors such as Cycl

SUBMITTER: Schudrowitz N 

PROVIDER: S-EPMC5543511 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature