Unknown

Dataset Information

0

Sensitive Molecular Diagnostics for Cutaneous Leishmaniasis.


ABSTRACT: Rapid diagnosis of cutaneous leishmaniasis (CL) and identification of Leishmania species is highly important for the disease management. In Israel, CL is caused mainly by Leishmania major and Leishmania tropica species.We established an easy to handle point of care lesion-swabbing, combined with a highly sensitive multiplex real time PCR (multiplex qPCR) for accurate and rapid diagnosis of Leishmania species.Using three probes: one general for: Leishmania species, and two specific for L major, and L tropica, we screened 1783 clinical samples collected during two years. Leishmania species was found in 1086 individuals, 1008 L major, and 70 L tropica. Eight samples positive for Leishmania species only, were further tested using a second set of multiplex qPCR developed, and were found positive for Leishmania braziliensis and Leishmania infantum/donovani (2 and 6 samples, concomitantly).Taken together, the test enabled diagnostics and better treatment of Leishmania infections from the Old World (1078 samples) and the New World (8 samples), and the subtyping of the dominant strains in the region, as well as in returning travelers'.

SUBMITTER: Sagi O 

PROVIDER: S-EPMC5543577 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

altmetric image

Publications

Sensitive Molecular Diagnostics for Cutaneous Leishmaniasis.

Sagi Orli O   Berkowitz Anat A   Codish Shlomi S   Novack Victor V   Rashti Aviv A   Akad Fouad F   Shemer-Avni Yonat Y  

Open forum infectious diseases 20170224 2


<h4>Background</h4>Rapid diagnosis of cutaneous leishmaniasis (CL) and identification of <i>Leishmania</i> species is highly important for the disease management. In Israel, CL is caused mainly by <i>Leishmania major</i> and <i>Leishmania tropica</i> species.<h4>Methods</h4>We established an easy to handle point of care lesion-swabbing, combined with a highly sensitive multiplex real time PCR (multiplex qPCR) for accurate and rapid diagnosis of <i>Leishmania</i> species.<h4>Results</h4>Using thr  ...[more]

Similar Datasets

| S-EPMC5605087 | biostudies-literature
2016-10-11 | MSV000080239 | GNPS
| S-EPMC6836376 | biostudies-literature
| S-EPMC6838665 | biostudies-literature
2012-10-24 | E-GEOD-33601 | biostudies-arrayexpress
| S-EPMC4887043 | biostudies-literature
| S-EPMC2942841 | biostudies-literature
2012-10-24 | GSE33601 | GEO
| S-EPMC10787922 | biostudies-literature
| S-EPMC4207691 | biostudies-literature