High-resolution hyperpolarized in vivo metabolic ¹³C spectroscopy at low magnetic field (48.7mT) following murine tail-vein injection.

ABSTRACT: High-resolution ¹³C NMR spectroscopy of hyperpolarized succinate-1-¹³C-2,3-d₂ is reported in vitro and in vivo using a clinical-scale, biplanar (80cm-gap) 48.7mT permanent magnet with a high homogeneity magnetic field. Non-localized ¹³C NMR spectra were recorded at 0.52MHz resonance frequency over the torso of a tumor-bearing mouse every 2s. Hyperpolarized ¹³C NMR signals with linewidths of ∼3Hz (corresponding to ∼6ppm) were recorded in vitro (2mL in a syringe) and in vivo (over a mouse torso). Comparison of the full width at half maximum (FWHM) for ¹³C NMR spectra acquired at 48.7mT and at 4.7T in a small-animal MRI scanner demonstrates a factor of ∼12 improvement for the ¹³C resonance linewidth attainable at 48.

SUBMITTER: Coffey AM 

PROVIDER: S-EPMC5544012 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature