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Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3.

ABSTRACT: The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for P. stutzeri NA3 and A. baumannii MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. P. stutzeri NA3 and A. baumannii MN3 produced 3.81 and 4.68 g/L of BS, respectively. Gas chromatography mass spectrometry confirmed that BS was mainly composed of fatty acids. Furthermore, the role of the degradative enzymes, alkane hydroxylase, alcohol dehydrogenase and laccase on biodegradation of crude oil are explained. Maximum biodegradation efficiency (BE) was recorded for mixed consortia (86%) followed by strain P. stutzeri NA3 (84%). Both bacterial strains were found to be vigorous biodegraders of crude oil than other biosurfactant-producing bacteria due to their enzyme production capabilities and our results suggests that the bacterial isolates can be used for effective degradation of crude oil within short time periods.

SUBMITTER: Parthipan P

PROVIDER: S-EPMC5545984 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3.

Parthipan Punniyakotti P Elumalai Punniyakotti P Sathishkumar Kuppusamy K Sabarinathan Devaraj D Murugan Kadarkarai K Benelli Giovanni G Rajasekar Aruliah A

3 Biotech 20170807 5

The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for P. stutzeri NA3 and A. baumannii MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. P. stutzeri NA3 and A. baumannii MN3 produced 3.81 and 4.68 g/L of BS, respec ...[more]

PMID: 28794933

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Project description:AimTo study immunogenicity of Pseudomonas N terminal flagellin as an adjuvant for Acinetobacter baumannii (A. baumannii) biofilm associated protein (Bap).MethodsThe N terminal flagellin gene was amplified. The pET28a (+) and polymerase chain reaction products were digested with HindIII and EcoR I. The ligation of N terminal flagellin into pET28a (‏+) was performed using T4 DNA ligase and was then transformed into Escherichia coli BL21 (DE3) as a suitable expression host. pET28a (‏+) vector harboring a conserved region of Bap from our previous work was used. The recombinant proteins were expressed, analyzed by SDS-PAGE method and was purified by affinity chromatography with His-Tag residues followed by confirmation with western blotting. Mice were immunized with recombinant N terminal flagellin and Bap subunits. The immunized animals were intranasally (i.n) challenged with A. baumannii and Pseudomonas aeruginosa (P. aeruginosa).ResultsThe flagellin enhanced the immunogenicity of Bap causing an increase in specific IgG titers in serum (P < 0.001). Internal organs, i.e., liver, lung and spleen of the Bap-Flagellin immunized group challenged with A. baumannii showed significantly lower bacterial load compared to the control group. The bacterial loads were studied in internal organs. A. baumannii infected immunized animals with Bap-Flagellin exhibited internal organs with minor bacterial load while P. aeruginosa PAO1 infected group showed heavy bacterial load of (4.3 ± 0.12) × 10(6), (1.1 ± 0.01) × 10(6) and (2.2 ± 0.22) × 10(6) per gram of lungs, liver and spleen respectively. Bacterial loads were detected per gram of lungs, liver and spleen of the mice group immunized with Bap were (1.2 ± 0.06) × 10(7), (11.1 ± 0.041) × 10(5) and (3.6 ± 0.42) × 10(6) respectively. In vivo neutralization assay indicated that all experimental mice groups, except for Flagellin administered group was significantly (P < 0.05) protected against A. baumannii.ConclusionThese results demonstrate that P. aeruginosa Flagellin as an adjuvant for Bap A. baumannii could be a useful model to evaluate new vaccine against A. baumannii.

Dataset Information

Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3.

Publications

Biosurfactant and enzyme mediated crude oil degradation by <i>Pseudomonas stutzeri</i> NA3 and <i>Acinetobacter baumannii</i> MN3.

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