Ontology highlight
ABSTRACT:
SUBMITTER: Xie X
PROVIDER: S-EPMC5546871 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Xie Xinqiang X Garg Ashish A Khosla Chaitan C Cane David E DE
Journal of the American Chemical Society 20170707 28
Dehydratase (DH) domains of cryptic function are often found in polyketide synthase (PKS) modules that produce epimerized (2S)-2-methyl-3-ketoacyl-ACP (acyl carrier protein) intermediates. A combination of tandem equilibrium isotope exchange (EIX) and a newly developed Tandem Modules Epimerase assay revealed the intrinsic epimerase activity of NanDH1 and NanDH5, from modules 1 and 5, respectively, of the nanchangmycin (1) PKS as well of NigDH1, from module 1 of the nigericin (3) PKS. Unexpectedl ...[more]