Ontology highlight
ABSTRACT:
SUBMITTER: Liu TY
PROVIDER: S-EPMC5546878 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Liu Tzu-Yu TY Huang Hector H HH Wheeler Diamond D Xu Yichen Y Wells James A JA Song Yun S YS Wiita Arun P AP
Cell systems 20170531 6
Ribosome profiling is a widespread tool for studying translational dynamics in human cells. Its central assumption is that ribosome footprint density on a transcript quantitatively reflects protein synthesis. Here, we test this assumption using pulsed-SILAC (pSILAC) high-accuracy targeted proteomics. We focus on multiple myeloma cells exposed to bortezomib, a first-line chemotherapy and proteasome inhibitor. In the absence of drug effects, we found that direct measurement of protein synthesis by ...[more]