Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland.

ABSTRACT: Transporters involved in bile acid (BA) handling by the mammary gland are poorly understood. Here we have investigated the role of ABC proteins in blood-milk BA traffic and its sensitivity to maternal cholestasis. BA concentrations in rat and mouse serum were higher than in milk. BA profiles in both fluids were also different. In mammary gland, mRNA levels of ABC pumps transporting BAs were high for Bcrp, less abundant for Mrp1, Mrp3 and Mrp4 and negligible for Bsep and Mrp2. Milk BA concentrations were lower in Abcg2 ^-/- than in wild-type mice. Taurocholate administration (5 µmol, i.p.) increased 20-fold BA concentrations in serum, but only moderately in milk, even in Abcg2 ^-/- mice. Bile duct ligation (BDL) in pregnant rats markedly increased serum BA concentrations

SUBMITTER: Blazquez AMG 

PROVIDER: S-EPMC5547141 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature