Ontology highlight
ABSTRACT:
SUBMITTER: Rubin AF
PROVIDER: S-EPMC5547491 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Genome biology 20170807 1
Deep mutational scanning is a widely used method for multiplex measurement of functional consequences of protein variants. We developed a new deep mutational scanning statistical model that generates error estimates for each measurement, capturing both sampling error and consistency between replicates. We apply our model to one novel and five published datasets comprising 243,732 variants and demonstrate its superiority in removing noisy variants and conducting hypothesis testing. Simulations sh ...[more]