Ontology highlight
ABSTRACT:
SUBMITTER: Barral J
PROVIDER: S-EPMC5548145 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Bio-protocol 20170601 12
We studied a network of cortical neurons in culture and developed an innovative optical device to stimulate optogenetically a large neuronal population with both spatial and temporal precision. We first describe how to culture primary neurons expressing channelrhodopsin. We then detail the optogenetic setup based on the workings of a fast Digital Light Processing (DLP) projector. The setup is able to stimulate tens to hundreds neurons with independent trains of light pulses that evoked action po ...[more]