Dataset Information

Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability.

ABSTRACT: E3 ligases play significant roles in plant stress tolerance by targeting specific substrate proteins for post-translational modification. In a previous study, we cloned TaPUB1 from Triticum aestivum L., which encodes a U-box E3 ligase. Real-time polymerase chain reaction revealed that the gene was up-regulated under drought stress. To investigate the function of TaPUB1 in the response of plants to drought, we generated transgenic Nicotiana benthamiana (N. benthamiana) plants constitutively expressing TaPUB1 under the CaMV35S promoter. Compared to wild type (WT), the transgenic plants had higher germination and seedling survival rates as well as higher photosynthetic rate and water retention, suggesting that the overexpression of TaPUB1 enhanced the drought tolerance of the TaPUB1 overexpressing (OE) plants. Moreover, less accumulation of reactive oxygen species (ROS) and stronger antioxidant capacity were detected in the OE plants than in the WT plants. To characterize the mechanisms involved, methyl viologen (MV) was used to induce oxidative stress conditions and we identified the functions of this gene in the plant tolerance to oxidative stress. Our results suggest that TaPUB1 positively modulates plant drought stress resistance potential by improving their antioxidant capacity.

SUBMITTER: Zhang G

PROVIDER: S-EPMC5548723 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability.

Zhang Guangqiang G Zhang Meng M Zhao Zhongxian Z Ren Yuanqing Y Li Qinxue Q Wang Wei W

Scientific reports 20170808 1

E3 ligases play significant roles in plant stress tolerance by targeting specific substrate proteins for post-translational modification. In a previous study, we cloned TaPUB1 from Triticum aestivum L., which encodes a U-box E3 ligase. Real-time polymerase chain reaction revealed that the gene was up-regulated under drought stress. To investigate the function of TaPUB1 in the response of plants to drought, we generated transgenic Nicotiana benthamiana (N. benthamiana) plants constitutively expre ...[more]

PMID: 28790447

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Project description:Maintenance of plant physiological functions under drought stress is normally considered a positive feature as it indicates sustained plant health and growth. This study was conducted to investigate whether plant growth-promoting rhizobacteria (PGPR) Bacillus subtilis HAS31 has potential to maintain potato growth and yield under drought stress. We analyzed trends of chlorophyll concentration, photosynthesis process, relative water content, osmolytes, antioxidants enzymes and oxidative stress, relative growth rate, tuber and aboveground biomass production in two potato varieties, Santae (drought-tolerant) and PRI-Red (drought-sensitive). Plants of both genotypes were treated with 100 g of HAS31 inoculant at 10 days after germination and exposed to different soil relative water contents (SRWC), including 80?±?5% (well watered), 60?±?5% (moderate stress) and 40?±?5% SRWC (severe stress) for 7 days at tuber initiation stage (30 days after germination). The drought stress reduced plant relative growth rate, biomass production, leaf area, number of leaves and tubers, tuber weight, and final yield. The drought-stressed plants showed decline in chlorophyll contents, membrane stability, leaf relative water contents and photosynthetic rate. Under drought stress, enzymatic activity of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD), contents of total soluble sugars, soluble proteins and proline increased. The application of PGPR reduced the impact of drought and maintained higher growth and physio-chemical traits of the plants. The plants with PGPR application showed higher relative growth rate, dry matter production, leaf area, number of tubers, tuber weight and yield as compared to plants without PGPR. The PGPR-HAS31 treated plants maintained higher photosynthetic process, contents of chlorophyll, soluble proteins, total soluble sugars, and enzymatic activities of CAT, POD and SOD as compared to plants without PGPR. The results of the study suggest that plant growth regulators have ability to sustain growth and yield of potato under drought stress by maintaining physiological functions of the plants.

Project description:BackgroundPlants are sessile organisms and are unable to relocate to favorable locations under extreme environmental conditions. Hence they have no choice but to acclimate and eventually adapt to the severe conditions to ensure their survival. As traditional methods of bolstering plant defense against stressful conditions come to their biological limit, we require newer methods that can allow us to strengthen plants' internal defense mechanism. These factors motivated us to look into the genetic networks of plants. The WRKY transcription factors are well known for their role in plant defense against biotic stresses, but recent studies have shed light on their activities against abiotic stresses such as drought. We modeled this network of WRKY transcription factors using Bayesian networks and applied inference algorithm to find the best regulators of drought response. Biologically intervening (activating/inhibiting) these regulators can bolster the defense response of plants against droughts.ResultWe used real world data from the NCBI GEO database and synthetic data generated from dependencies in the Bayesian network to learn the network parameters. These parameters were estimated using both a Bayesian and a frequentist approach. The two sets of parameters were used in a utility-based inference algorithm to determine the best regulator of plant drought response in the WRKY transcription factor network.ConclusionOur analysis revealed that activating the transcription factor WRKY18 had the highest likelihood of inducing drought response among all the other elements of the WRKY transcription factor network. Our observation was also supported by biological literature, as WRKY18 is known to regulate drought responsive genes positively. We also found that activating the protein complex WRKY60-60 had the second highest likelihood of inducing drought defense response. Consistent with the existing biological literature, we also found the transcription factor WRKY40 and the protein complex WRKY40-40 to suppress drought response.

Dataset Information

Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability.

Publications

Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability.

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