An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells.
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ABSTRACT:
SUBMITTER: Tcw J
PROVIDER: S-EPMC5550034 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
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An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells.
Tcw Julia J Wang Minghui M Pimenova Anna A AA Bowles Kathryn R KR Hartley Brigham J BJ Lacin Emre E Machlovi Saima I SI Abdelaal Rawan R Karch Celeste M CM Phatnani Hemali H Slesinger Paul A PA Zhang Bin B Goate Alison M AM Brennand Kristen J KJ
Stem cell reports 20170727 2
Growing evidence implicates the importance of glia, particularly astrocytes, in neurological and psychiatric diseases. Here, we describe a rapid and robust method for the differentiation of highly pure populations of replicative astrocytes from human induced pluripotent stem cells (hiPSCs), via a neural progenitor cell (NPC) intermediate. We evaluated this protocol across 42 NPC lines (derived from 30 individuals). Transcriptomic analysis demonstrated that hiPSC-astrocytes from four individuals ...[more]
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