Unknown

Dataset Information

0

The adaptive landscape of wildtype and glycosylation-deficient populations of the industrial yeast Pichia pastoris.


ABSTRACT: BACKGROUND:The effects of long-term environmental adaptation and the implications of major cellular malfunctions are still poorly understood for non-model but biotechnologically relevant species. In this study we performed a large-scale laboratory evolution experiment with 48 populations of the yeast Pichia pastoris in order to establish a general adaptive landscape upon long-term selection in several glucose-based growth environments. As a model for a cellular malfunction the implications of OCH1 mannosyltransferase knockout-mediated glycosylation-deficiency were analyzed. RESULTS:In-depth growth profiling of evolved populations revealed several instances of genotype-dependent growth trade-off/cross-benefit correlations in non-evolutionary growth conditions. On the genome level a high degree of mutational convergence was observed among independent populations. Environment-dependent mutational hotspots were related to osmotic stress-, Rim - and cAMP signaling pathways. In agreement with the observed growth phenotypes, our data also suggest diverging compensatory mutations in glycosylation-deficient populations. High osmolarity glycerol (HOG) pathway loss-of-functions mutations, including genes such as SSK2 and SSK4, represented a major adaptive strategy during environmental adaptation. However, genotype-specific HOG-related mutations were predominantly observed in opposing environmental conditions. Surprisingly, such mutations emerged during salt stress adaptation in OCH1 knockout populations and led to growth trade-offs in non-adaptive conditions that were distinct from wildtype HOG-mutants. Further environment-dependent mutations were identified for a hitherto uncharacterized species-specific Gal4-like transcriptional regulator involved in environmental sensing. CONCLUSION:We show that metabolic constraints such as glycosylation-deficiency can contribute to evolution on the molecular level, even in non-diverging growth environments. Our dataset suggests universal adaptive mechanisms involving cellular stress response and cAMP/PKA signaling but also the existence of highly species-specific strategies involving unique transcriptional regulators, improving our biological understanding of distinct Ascomycetes species.

SUBMITTER: Moser JW 

PROVIDER: S-EPMC5553748 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

The adaptive landscape of wildtype and glycosylation-deficient populations of the industrial yeast Pichia pastoris.

Moser Josef W JW   Wilson Iain B H IBH   Dragosits Martin M  

BMC genomics 20170810 1


<h4>Background</h4>The effects of long-term environmental adaptation and the implications of major cellular malfunctions are still poorly understood for non-model but biotechnologically relevant species. In this study we performed a large-scale laboratory evolution experiment with 48 populations of the yeast Pichia pastoris in order to establish a general adaptive landscape upon long-term selection in several glucose-based growth environments. As a model for a cellular malfunction the implicatio  ...[more]

Similar Datasets

| S-EPMC8287956 | biostudies-literature
| S-EPMC154291 | biostudies-literature
| S-EPMC8364938 | biostudies-literature
| S-EPMC4111905 | biostudies-literature
| S-EPMC9754263 | biostudies-literature
| S-EPMC7008030 | biostudies-literature
| S-EPMC6127371 | biostudies-literature
| S-EPMC4147080 | biostudies-literature
| S-EPMC10540378 | biostudies-literature
| S-EPMC3064196 | biostudies-literature