Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene.
Ontology highlight
ABSTRACT: Clostridium tyrobutyricum ATCC25755 has been reported as being able to produce significant quantities of hydrogen. In this study, the exo-inulinase encoding gene cloned from Paenibacillus polymyxa SC-2 was into the expression plasmid pSY6 and expressed in the cells of C. tyrobutyricum. The engineered C. tyrobutyricum strain efficiently fermented the inulin-type carbohydrates from Jerusalem artichoke, without any pretreatment being necessary for the production of hydrogen. A comparatively high hydrogen yield (3.7?mol/mol inulin-type sugar) was achieved after 96?h in a batch process with simultaneous saccharification and fermentation (SSF), with an overall volumetric productivity rate of 620?±?60?mL/h/L when the initial total sugar concentration of the inulin extract was increased to 100?g/L. Synthesis of inulinase in the batch SSF culture was closely associated with strain growth until the end of the exponential phase, reaching a maximum activity of 28.4?±?0.26?U/mL. The overall results show that the highly productive and abundant biomass crop Jerusalem artichoke can be a good substrate for hydrogen production, and that the application of batch SSF for its conversion has the potential to become a cost-effective process in the near future.
SUBMITTER: Jiang L
PROVIDER: S-EPMC5554141 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
ACCESS DATA