Ontology highlight
ABSTRACT:
SUBMITTER: Horii T
PROVIDER: S-EPMC5554182 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Scientific reports 20170811 1
Conditional knockout using Cre/lox is essential for functional analysis of genes. CRISPR/Cas in combination with two sets of guide RNAs and a single-stranded oligonucleotide enables simultaneous insertion of two lox sequences. However, this method induces double-strand breaks at two sites on the same chromosome, which causes an undesirable chromosomal deletion and reduces the flanked lox (flox) rate. To solve this problem, we investigated a method that sequentially introduces each lox sequence a ...[more]