Ontology highlight
ABSTRACT:
SUBMITTER: Kappe SH
PROVIDER: S-EPMC55549 | biostudies-literature | 2001 Aug
REPOSITORIES: biostudies-literature
Kappe S H SH Gardner M J MJ Brown S M SM Ross J J Matuschewski K K Ribeiro J M JM Adams J H JH Quackenbush J J Cho J J Carucci D J DJ Hoffman S L SL Nussenzweig V V
Proceedings of the National Academy of Sciences of the United States of America 20010807 17
Most studies of gene expression in Plasmodium have been concerned with asexual and/or sexual erythrocytic stages. Identification and cloning of genes expressed in the preerythrocytic stages lag far behind. We have constructed a high quality cDNA library of the Plasmodium sporozoite stage by using the rodent malaria parasite P. yoelii, an important model for malaria vaccine development. The technical obstacles associated with limited amounts of RNA material were overcome by PCR-amplifying the tra ...[more]