Ontology highlight
ABSTRACT:
SUBMITTER: Cheung MS
PROVIDER: S-EPMC555696 | biostudies-literature | 2005 Mar
REPOSITORIES: biostudies-literature
Cheung Margaret S MS Klimov Dmitri D Thirumalai D D
Proceedings of the National Academy of Sciences of the United States of America 20050321 13
The presence of macromolecules in cells geometrically restricts the available space for poplypeptide chains. To study the effects of macromolecular crowding on folding thermodynamics and kinetics, we used an off-lattice model of the all-beta-sheet WW domain in the presence of large spherical particles whose interaction with the polypeptide chain is purely repulsive. At all volume fractions, phi(c), of the crowding agents the stability of the native state is enhanced. Remarkably, the refolding ra ...[more]