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Iterative expansion microscopy.

ABSTRACT: We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by ?4.5× in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded ?20×. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens ?4.5 × 4.5, or ?20×, and enables ?25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.

SUBMITTER: Chang JB

PROVIDER: S-EPMC5560071 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Iterative expansion microscopy.

Chang Jae-Byum JB Chen Fei F Yoon Young-Gyu YG Jung Erica E EE Babcock Hazen H Kang Jeong Seuk JS Asano Shoh S Suk Ho-Jun HJ Pak Nikita N Tillberg Paul W PW Wassie Asmamaw T AT Cai Dawen D Boyden Edward S ES

Nature methods 20170417 6

We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by ∼4.5× in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded ∼20×. After preliminary expansion a second swellable polymer ...[more]

PMID: 28417997

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