Dataset Information

Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos.

ABSTRACT: Pesticides in urban runoff are a major source of pollutants in aquatic ecosystems. Fipronil, a phenylpyrazole insecticide, found in structural pest control products, turf grass control, and home pet flea medication, has recently increased in use and is commonly detected in urban runoff. However, little is known about the effects of fipronil on aquatic organisms at early developmental stages. Here, we evaluated toxicity of fipronil to embryos of Japanese Medaka (Oryzias latipes, Qurt strain) using a high-throughput 96-well plate toxicity test. Male and female embryos (<6 h post fertilization) were exposed to concentrations of fipronil ranging from 0.1 to 910 ?g L-1 for 14 days or until hatching. Embryos were subjected to gross and microscopic examinations of developmental adverse effects as well as transcriptome analysis using RNA-seq. Results indicated a positive dose-response in reduced hatching success, increased gross deformity (tail curvature) at a lowest-observed-effect concentration (LOEC) of 200 ?g L-1 and delayed hatching (?1 day at the highest concentration, LOEC = 600 ?g L-1). The transcriptome analysis indicated that fipronil exposure enhanced expression of titin and telethonin, which are responsible for muscle development. It is therefore possible that the formation of a tail curvature is due to asymmetrical overgrowth of muscle. Our results indicate that sub-lethal effects occur in embryonic stages of an aquatic vertebrate following exposure to high concentrations of fipronil, although no adverse effects at the highest published environmentally relevant concentration (6.3 ?g L-1) were observed.

SUBMITTER: Wagner SD

PROVIDER: S-EPMC5560107 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos.

Wagner Scott D SD Kurobe Tomofumi T Hammock Bruce G BG Lam Chelsea H CH Wu Gary G Vasylieva Natalia N Gee Shirley J SJ Hammock Bruce D BD Teh Swee J SJ

Chemosphere 20161003

Pesticides in urban runoff are a major source of pollutants in aquatic ecosystems. Fipronil, a phenylpyrazole insecticide, found in structural pest control products, turf grass control, and home pet flea medication, has recently increased in use and is commonly detected in urban runoff. However, little is known about the effects of fipronil on aquatic organisms at early developmental stages. Here, we evaluated toxicity of fipronil to embryos of Japanese Medaka (Oryzias latipes, Qurt strain) usin ...[more]

PMID: 27710886

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Project description:The datasets of this article present the experimental parameters resulting from the synthesis and characterization of graphene oxide (GO) using scanning and transmission electron microscopy (SEM, TEM) and spectrophotometric (FTIR, AFM, EDX) methods, and the assessment of its toxicological and endocrine-disrupting effects on the Japanese medaka fish by acute toxicity testing, and histopathological evaluations. These datasets support the article "Reproductive and Developmental Effects of Graphene Oxide on Japanese Medaka (Oryzias latipes)". GO synthesis was performed following the modified Hummer's method. Its particle diameter and zeta potential were determined using Zeta Sizer Nano ZS analyzer, and characterized by SEM and TEM. After 5 min sonication in water, GO (25-200 µg/g) was injected intraperitoneally to the reproductively active male and female fish maintained as a breeding pair (one male, one female) in 500 mL balanced salt solution (BSS) in glass jars under standard laboratory conditions (25±1 °C; 16L:8D light cycle). The control fish were injected with water. The maximum volume of the injected material is 1 µL/10 mg body weight. To avoid movement, during injection the fish were briefly anesthetized in MS 222 (100 mg/L) and after injection transferred to BSS for recovery. LD50 values of GO related to fish mortality were determined from the linear regression analysis using a software program. Reproductive activities (fecundity) were determined by daily collection of eggs 7 days before and 21 days after injection from a breeding pair and expressed as percent eggs laid every day post-injection relative to the average (mean of 7 days) eggs laid prior to injection. Developmental abnormalities of the embryos were assessed by culturing the collected fertilized eggs in ERM for a maximum period of 14 day-post fertilization (dpf). The fish that survived after 21days post-injection were sacrificed and the entire fish excluding post-anal tail were cut into three small pieces and fixed in 4% paraformaldehyde containing 0.05% Tween 20. Histopathological evaluations of gonads (ovary and testis), liver, and kidneys were made in 5 µm thick sections stained mainly on hematoxylin and eosin (HE) following the guidelines published by OECD. The Photomicrographs of the sections were made using Olympus B-max 40 microscope attached to a camera with Q-capture Pro 7 software or in Nikon Eclipse 50i microscope attached to Nikon DS-Fi1 camera. Four types of follicles in the stromal compartments of the ovary, perinucleolar (PNO), cortical alveolar (CAO), early vitellogenic (EVO) and late vitellogenic (LVO) were considered as differentiating, and the post ovulatory and atretic follicles were considered as degenerating follicles, and counted in an entire section made through four different regions (anterior, upper middle, lower middle, and anal) of the ovary. The follicular data were expressed as percent follicles (individual follicles or differentiating or degenerating) or as the ratio of differentiating and degenerating follicles found in that particular region of the ovary. The data were analyzed either by one- or two-way ANOVA followed by post-hoc Tukey's multiple comparison test and expressed as means ±SEM.

Dataset Information

Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos.

Publications

Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos.

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