Ontology highlight
ABSTRACT:
SUBMITTER: Edwards T
PROVIDER: S-EPMC5560405 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Diagnostic microbiology and infectious disease 20170608 1
Chikungunya was introduced into the Americas in 2015 causing a pandemic across the continent. Testing during the acute phase of infection relies on qRT-PCR, but available assays have a number of limitations. A qRT-PCR assay specific to the chikungunya E1 gene was designed using sequence data from contemporary strains. A probit analysis established the 95% limit of detection as 19.6 copies per reaction. We compared the assay with a US Centers for Disease Control (CDC) chikungunya qRT-PCR as the r ...[more]