Dataset Information

Functional variants in the sucrase-isomaltase gene associate with increased risk of irritable bowel syndrome.

ABSTRACT:

Objective

IBS is a common gut disorder of uncertain pathogenesis. Among other factors, genetics and certain foods are proposed to contribute. Congenital sucrase-isomaltase deficiency (CSID) is a rare genetic form of disaccharide malabsorption characterised by diarrhoea, abdominal pain and bloating, which are features common to IBS. We tested sucrase-isomaltase (SI) gene variants for their potential relevance in IBS.

Design

We sequenced SI exons in seven familial cases, and screened four CSID mutations (p.Val557Gly, p.Gly1073Asp, p.Arg1124Ter and p.Phe1745Cys) and a common SI coding polymorphism (p.Val15Phe) in a multicentre cohort of 1887 cases and controls. We studied the effect of the 15Val to 15Phe substitution on SI function in vitro. We analysed p.Val15Phe genotype in relation to IBS status, stool frequency and faecal microbiota composition in 250 individuals from the general population.

Results

CSID mutations were more common in patients than asymptomatic controls (p=0.074; OR=1.84) and Exome Aggregation Consortium reference sequenced individuals (p=0.020; OR=1.57). 15Phe was detected in 6/7 sequenced familial cases, and increased IBS risk in case-control and population-based cohorts, with best evidence for diarrhoea phenotypes (combined p=0.00012; OR=1.36). In the population-based sample, 15Phe allele dosage correlated with stool frequency (p=0.026) and Parabacteroides faecal microbiota abundance (p=0.0024). The SI protein with 15Phe exhibited 35% reduced enzymatic activity in vitro compared with 15Val (p<0.05).

Conclusions

SI gene variants coding for disaccharidases with defective or reduced enzymatic activity predispose to IBS. This may help the identification of individuals at risk, and contribute to personalising treatment options in a subset of patients.

SUBMITTER: Henstrom M

PROVIDER: S-EPMC5563477 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Publications

Functional variants in the sucrase-isomaltase gene associate with increased risk of irritable bowel syndrome.

Henström Maria M Diekmann Lena L Bonfiglio Ferdinando F Hadizadeh Fatemeh F Kuech Eva-Maria EM von Köckritz-Blickwede Maren M Thingholm Louise B LB Zheng Tenghao T Assadi Ghazaleh G Dierks Claudia C Heine Martin M Philipp Ute U Distl Ottmar O Money Mary E ME Belheouane Meriem M Heinsen Femke-Anouska FA Rafter Joseph J Nardone Gerardo G Cuomo Rosario R Usai-Satta Paolo P Galeazzi Francesca F Neri Matteo M Walter Susanna S Simrén Magnus M Karling Pontus P Ohlsson Bodil B Schmidt Peter T PT Lindberg Greger G Dlugosz Aldona A Agreus Lars L Andreasson Anna A Mayer Emeran E Baines John F JF Engstrand Lars L Portincasa Piero P Bellini Massimo M Stanghellini Vincenzo V Barbara Giovanni G Chang Lin L Camilleri Michael M Franke Andre A Naim Hassan Y HY D'Amato Mauro M

Gut 20161121 2

<h4>Objective</h4>IBS is a common gut disorder of uncertain pathogenesis. Among other factors, genetics and certain foods are proposed to contribute. Congenital sucrase-isomaltase deficiency (CSID) is a rare genetic form of disaccharide malabsorption characterised by diarrhoea, abdominal pain and bloating, which are features common to IBS. We tested sucrase-isomaltase (<i>SI</i>) gene variants for their potential relevance in IBS.<h4>Design</h4>We sequenced <i>SI</i> exons in seven familial case ...[more]

PMID: 27872184

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Project description:Corticotropin-releasing hormone (CRH) plays an important role in the pathophysiology of irritable bowel syndrome (IBS) and regulates the stress response through two CRH receptors (R1 and R2). Previously, we reported that a CRHR1 gene polymorphism (rs110402, rs242924, and rs7209436) and haplotypes were associated with IBS. However, the association between the CRHR2 gene and IBS was not investigated. We tested the hypothesis that genetic polymorphisms and haplotypes of CRHR2 are associated with IBS pathophysiology and negative emotion in IBS patients.A total of 142 IBS patients and 142 healthy controls participated in this study. Seven single nucleotide polymorphisms (SNPs) of the CRHR2 gene (rs4722999, rs3779250, rs2240403, rs2267710, rs2190242, rs2284217, and rs2284220) were genotyped. Subjects' psychological states were evaluated using the Perceived-Stress Scale, the State-Trait Anxiety Inventory, and the Self-Rating Depression Scale.We found that rs4722999 and rs3779250, located in intronic region, were associated with IBS in terms of genotype frequency (rs4722999: P = 0.037; rs3779250: P = 0.017) and that the distribution of the major allele was significantly different between patients and controls. There was a significant group effect (controls vs. IBS), and a CRHR2 genotype effect was observed for three psychological scores, but the interaction was not significant. We found a haplotype of four SNPs (rs4722999, rs3779250, rs2240403, and rs2267710) and two SNPs (rs2284217 and rs2284220) in strong linkage disequilibrium (D' > 0.90). We also found that haplotypes of the CRHR2 gene were significantly different between IBS patients and controls and that they were associated with negative emotion.Our findings support the hypothesis that genetic polymorphisms and haplotypes of CRHR2 are related to IBS. In addition, we found associations between CRHR2 genotypes and haplotypes and negative emotion in IBS patients and controls. Further studies on IBS and the CRH system are warranted.

Dataset Information

Functional variants in the sucrase-isomaltase gene associate with increased risk of irritable bowel syndrome.

Objective

Design

Results

Conclusions

Publications

Functional variants in the sucrase-isomaltase gene associate with increased risk of irritable bowel syndrome.

Similar Datasets

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