Characterization of Rebound Depolarization in Neurons of the Rat Medial Geniculate Body In Vitro.

ABSTRACT: Rebound depolarization (RD) is a response to the offset from hyperpolarization of the neuronal membrane potential and is an important mechanism for the synaptic processing of inhibitory signals. In the present study, we characterized RD in neurons of the rat medial geniculate body (MGB), a nucleus of the auditory thalamus, using whole-cell patch-clamp and brain slices. RD was proportional in strength to the duration and magnitude of the hyperpolarization; was effectively blocked by Ni(2+) or Mibefradil; and was depressed when the resting membrane potential was hyperpolarized by blocking hyperpolarization-activated cyclic nucleotide-gated (HCN) channels with ZD7288 or by activating G-protein-gated inwardly-rectifying K(+) (GIRK) channels with baclofen. Our results demonstrated that RD in MGB neurons, which is carried by T-type Ca(2+) channels, is critically regulated by HCN channels and likely by GIRK channels.

SUBMITTER: Wang XX 

PROVIDER: S-EPMC5563751 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature