Unknown

Dataset Information

0

Superresolution expansion microscopy reveals the three-dimensional organization of the Drosophila synaptonemal complex.


ABSTRACT: The synaptonemal complex (SC), a structure highly conserved from yeast to mammals, assembles between homologous chromosomes and is essential for accurate chromosome segregation at the first meiotic division. In Drosophila melanogaster, many SC components and their general positions within the complex have been dissected through a combination of genetic analyses, superresolution microscopy, and electron microscopy. Although these studies provide a 2D understanding of SC structure in Drosophila, the inability to optically resolve the minute distances between proteins in the complex has precluded its 3D characterization. A recently described technology termed expansion microscopy (ExM) uniformly increases the size of a biological sample, thereby circumventing the limits of optical resolution. By adapting the ExM protocol to render it compatible with structured illumination microscopy, we can examine the 3D organization of several known Drosophila SC components. These data provide evidence that two layers of SC are assembled. We further speculate that each SC layer may connect two nonsister chromatids, and present a 3D model of the Drosophila SC based on these findings.

SUBMITTER: Cahoon CK 

PROVIDER: S-EPMC5565445 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Superresolution expansion microscopy reveals the three-dimensional organization of the <i>Drosophila</i> synaptonemal complex.

Cahoon Cori K CK   Yu Zulin Z   Wang Yongfu Y   Guo Fengli F   Unruh Jay R JR   Slaughter Brian D BD   Hawley R Scott RS  

Proceedings of the National Academy of Sciences of the United States of America 20170731 33


The synaptonemal complex (SC), a structure highly conserved from yeast to mammals, assembles between homologous chromosomes and is essential for accurate chromosome segregation at the first meiotic division. In <i>Drosophila melanogaster</i>, many SC components and their general positions within the complex have been dissected through a combination of genetic analyses, superresolution microscopy, and electron microscopy. Although these studies provide a 2D understanding of SC structure in <i>Dro  ...[more]

Similar Datasets

| S-EPMC5474826 | biostudies-other
| S-EPMC6014096 | biostudies-literature
| S-EPMC8351745 | biostudies-literature
| S-EPMC3694795 | biostudies-literature
| S-EPMC7320163 | biostudies-literature
| S-EPMC5221576 | biostudies-literature
| S-EPMC4538611 | biostudies-literature
| S-EPMC5752594 | biostudies-literature
| S-EPMC7276552 | biostudies-literature
| S-EPMC8108526 | biostudies-literature