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Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.


ABSTRACT: Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4?×?2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which employed droplet microfluidics to conduct drug screens against suspended and adherent cancer cell lines, as well as cells dissociated from primary tumor of human patients. Single cells were dispersed in aqueous droplets and imaged within 24?hours of drug treatment to assess cell viability by ethidium homodimer 1 staining. Our results showed that 5 conditions could be screened for every 80,000 cells in one channel on our chip under current circumstances. Additionally, screening conditions have been adapted to both suspended and adherent cancer cells, giving versatility to potentially all types of cancers. Hence, this study provides a powerful tool for rapid, low-input drug screening of primary cancers within 24?hours after tumor resection from cancer patients. This paves the way for further technological advancement to cutting down sample size and increasing drug screening throughput in advent to personalized cancer therapy.

SUBMITTER: Wong AH 

PROVIDER: S-EPMC5567315 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.

Wong Ada Hang-Heng AH   Li Haoran H   Jia Yanwei Y   Mak Pui-In PI   Martins Rui Paulo da Silva RPDS   Liu Yan Y   Vong Chi Man CM   Wong Hang Cheong HC   Wong Pak Kin PK   Wang Haitao H   Sun Heng H   Deng Chu-Xia CX  

Scientific reports 20170822 1


Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 × 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which employed droplet microfluidics to conduct drug screens against suspended and adherent cancer cell lines, as well as cells dissociated from pr  ...[more]

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2017-10-06 | GSE104525 | GEO