Ontology highlight
ABSTRACT:
SUBMITTER: Han K
PROVIDER: S-EPMC5567838 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Han Kook K Tjaden Brian B Lory Stephen S
Nature microbiology 20161222
The first step in the post-transcriptional regulatory function of most bacterial small non-coding RNAs (sRNAs) is base pairing with partially complementary sequences of targeted transcripts. We present a simple method for identifying sRNA targets in vivo and defining processing sites of the regulated transcripts. The technique, referred to as global small non-coding RNA target identification by ligation and sequencing (GRIL-seq), is based on preferential ligation of sRNAs to the ends of base-pai ...[more]