Fine Epitope Mapping of Two Antibodies Neutralizing the Bordetella Adenylate Cyclase Toxin.

ABSTRACT: Adenylate cyclase toxin (ACT) is an important Bordetella pertussis virulence factor that is not included in current acellular pertussis vaccines. We previously demonstrated that immunization with the repeat-in-toxin (RTX) domain of ACT elicits neutralizing antibodies in mice and discovered the first two antibodies to neutralize ACT activities by occluding the receptor-binding site. Here, we fully characterize these antibodies and their epitopes. Both antibodies bind ACT with low nanomolar affinity and cross-react with ACT homologues produced by B. parapertussis and B. bronchiseptica. Antibody M1H5 binds B. pertussis RTX₇₅₁ ∼100-fold tighter than RTX₇₅₁ from the other two species, while antibody M2B10 has similar affinity for all three variants. To initially map the an

SUBMITTER: Wang X 

PROVIDER: S-EPMC5568097 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature