Ontology highlight
ABSTRACT:
SUBMITTER: Hansen KJ
PROVIDER: S-EPMC5568175 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Hansen Katrina J KJ Favreau John T JT Gershlak Joshua R JR Laflamme Michael A MA Albrecht Dirk R DR Gaudette Glenn R GR
Tissue engineering. Part C, Methods 20170627 8
Differentiation of human pluripotent stem cells into cardiomyocytes (hPS-CMs) holds promise for myocardial regeneration therapies, drug discovery, and models of cardiac disease. Potential cardiotoxicities may affect hPS-CM mechanical contraction independent of calcium signaling. Herein, a method using an image capture system is described to measure hPS-CM contractility and intracellular calcium concurrently, with high spatial and temporal resolution. The image capture system rapidly alternates b ...[more]