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Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity.


ABSTRACT: The innate immune components that modulate allergic contact hypersensitivity (CHS) responses are poorly defined. Using human skin from contact dermatitis patients and a mouse model of CHS, we find that hapten allergens disrupt the Arginase1 (Arg1) and inducible NO synthase (iNOS) dynamic in monocytes/macrophages (mono/M?), which renders those cells ineffectual in suppressing skin inflammation. Mice lacking Arg1 in M? develop increased CHS characterized by elevated ear thickening, mono/M?-dominated dermal inflammation, and increased iNOS and IL-6 expression compared with control mice. Treatment of Arg1flox/flox; LysMCre+/- mice with a selective NOS inhibitor or knockout of Nos2, encoding iNOS, significantly ameliorates CHS. Our findings suggest a critical role for Arg1 in mono/M? in suppressing CHS through dampening Nos2 expression. These results support that increasing Arg1 may be a potential therapeutic avenue in treating allergic contact dermatitis.

SUBMITTER: Suwanpradid J 

PROVIDER: S-EPMC5568483 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity.

Suwanpradid Jutamas J   Shih Michael M   Pontius Lauren L   Yang Bin B   Birukova Anastasiya A   Guttman-Yassky Emma E   Corcoran David L DL   Que Loretta G LG   Tighe Robert M RM   MacLeod Amanda S AS  

Journal of immunology (Baltimore, Md. : 1950) 20170726 5


The innate immune components that modulate allergic contact hypersensitivity (CHS) responses are poorly defined. Using human skin from contact dermatitis patients and a mouse model of CHS, we find that hapten allergens disrupt the Arginase1 (Arg1) and inducible NO synthase (iNOS) dynamic in monocytes/macrophages (mono/MΦ), which renders those cells ineffectual in suppressing skin inflammation. Mice lacking Arg1 in MΦ develop increased CHS characterized by elevated ear thickening, mono/MΦ-dominat  ...[more]

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