Ontology highlight
ABSTRACT:
SUBMITTER: Bak RO
PROVIDER: S-EPMC5568673 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Bak Rasmus O RO Porteus Matthew H MH
Cell reports 20170701 3
The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV) vectors to serve as donor template DNA during homologous recombination (HR). However, the maximum AAV packaging capacity of ∼4.5 kb limits the donor size. Here, we overcome this constraint by showing that two co-transduced AAV vectors can serve as donors during consecutive HR events for the integration of large transgenes. Importantly, the method involves a single ...[more]