Ontology highlight
ABSTRACT:
SUBMITTER: Peltier J
PROVIDER: S-EPMC5569036 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Peltier Johann J Shaw Helen A HA Wren Brendan W BW Fairweather Neil F NF
Scientific reports 20170823 1
Clostridium difficile is a gastrointestinal pathogen but how the bacterium colonises this niche is still little understood. Sortase enzymes covalently attach specific bacterial proteins to the peptidoglycan cell wall and are often involved in colonisation by pathogens. Here we show C. difficile proteins CD2537 and CD3392 are functional substrates of sortase SrtB. Through manipulation of the C-terminal regions of these proteins we show the SPKTG motif is essential for covalent attachment to the c ...[more]