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Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria.

ABSTRACT: Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared more alike than expected. Metabolic heat production rate was 2298?±?181?µW for M. smegmatis and 792?±?81?µW for M. phlei, while M. tuberculosis and M. bovis metabolic heat production rates were between these values. These small differences were further confirmed by similar oxygen consumption rates (3.3?±?0.2 nMole/s and 1.7?±?0.3 nMole/s for M. smegmatis and M. tuberculosis, respectively). These data suggest that the metabolic potential of slow-growing mycobacterial biofilms has been underestimated, particularly for pathogenic species.

SUBMITTER: Solokhina A

PROVIDER: S-EPMC5569076 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria.

Solokhina Anna A Brückner David D Bonkat Gernot G Braissant Olivier O

Scientific reports 20170823 1

Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared mo ...[more]

PMID: 28835629

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Dataset Information

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria.

Publications

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria.

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