Dataset Information

Diurnal variation of NMR based blood metabolites in calves fed a high plane of milk replacer: a pilot study.

ABSTRACT:

Background

Blood profiles have been used to monitor herd health status, diagnose disorders, and predict the risk of diseases in cattle and calves. Characterizing plasma metabolites in dairy calves could provide further insight into daily metabolic variations and the mechanisms that lead to metabolic diseases. In addition, by understanding physiological ranges of plasma metabolites relative to meal and the time of feeding in healthy animals, veterinarians can accurately diagnose abnormalities with a blood test. For diagnostic purposes, nuclear magnetic resonance (NMR) spectroscopy shows promise as a new and reliable method to determine a large number of blood metabolites simultaneously.

Results

Results demonstrated that the concentration of specific metabolites in plasma (i.e., lysine, isoleucine, leucine, tyrosine, glutamine, creatine, and 1-methylhistidine) fluctuated around meal times, while others (i.e., glutamic acid, methanol, formic acid, and acetic acid) maintained a stable temporal concentration. In addition to temporal changes in concentration, results also characterized differences for overall plasma metabolite concentrations; for example, methionine had the lowest (38 ?M) while glutamine had the highest concentration (239 ?M) amongst plasma AA. This is the first report describing how the plasma metabolome changes during 24-h period in young calves fed an elevated plane of milk replacer twice daily.

Conclusions

Data from this pilot study will help to establish reference standards for future metabolic diagnostics in dairy calves. In addition, this pilot study illustrated that feeding milk replacer may influence plasma metabolite concentrations. With the rapid implementation of blood metabolomics in monitoring animal health, it is then important to consider the time of feeding during the day when interpreting metabolomics analysis results.

SUBMITTER: Ghaffari MH

PROVIDER: S-EPMC5569568 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Publications

Diurnal variation of NMR based blood metabolites in calves fed a high plane of milk replacer: a pilot study.

Ghaffari Morteza H MH MacPherson Jayden A R JAR Berends Harma H Steele Michael A MA

BMC veterinary research 20170823 1

<h4>Background</h4>Blood profiles have been used to monitor herd health status, diagnose disorders, and predict the risk of diseases in cattle and calves. Characterizing plasma metabolites in dairy calves could provide further insight into daily metabolic variations and the mechanisms that lead to metabolic diseases. In addition, by understanding physiological ranges of plasma metabolites relative to meal and the time of feeding in healthy animals, veterinarians can accurately diagnose abnormali ...[more]

PMID: 28836978

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Dataset Information

Diurnal variation of NMR based blood metabolites in calves fed a high plane of milk replacer: a pilot study.

Background

Results

Conclusions

Publications

Diurnal variation of NMR based blood metabolites in calves fed a high plane of milk replacer: a pilot study.

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