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Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus.


ABSTRACT: Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.

SUBMITTER: Donchenko EK 

PROVIDER: S-EPMC5570366 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus.

Donchenko Ekaterina K EK   Pechnikova Evgeniya V EV   Mishyna Maryia Yu MY   Manukhova Tatiana I TI   Sokolova Olga S OS   Nikitin Nikolai A NA   Atabekov Joseph G JG   Karpova Olga V OV  

PloS one 20170824 8


Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and abse  ...[more]

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