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Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium.

ABSTRACT: A modified Pseudomonas aeruginosa strain capable of overexpressing the estA gene, an encoding gene for a membrane-bound esterase, was constructed and its rhamnolipid (RML) production was studied. Fermentations using wild-type (WT) and modified P. aeruginosa strains were conducted until exhaustion of glycerol in Medium Salt Production, using two different C/N ratios. At a C/N of 83.2, the modified strain produced up to 3.9 times more RMLs than the WT, yielding a maximum concentration of 14.62 g/L RML when measured by HPLC and 22 g/L by the orcinol assay. Cell-free supernatant from the modified strain reduced surface tension to 29.4 mN/m and had a CMC of 240 mg/L and CMD of 56.05. This is the first report on the construction of an estA-based recombinant strain for RML production.

SUBMITTER: Dobler L

PROVIDER: S-EPMC5570425 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium.

Dobler Leticia L de Carvalho Bruna Rocha BR Alves Wilber de Sousa WS Neves Bianca Cruz BC Freire Denise Maria Guimarães DMG Almeida Rodrigo Volcan RV

PloS one 20170824 8

A modified Pseudomonas aeruginosa strain capable of overexpressing the estA gene, an encoding gene for a membrane-bound esterase, was constructed and its rhamnolipid (RML) production was studied. Fermentations using wild-type (WT) and modified P. aeruginosa strains were conducted until exhaustion of glycerol in Medium Salt Production, using two different C/N ratios. At a C/N of 83.2, the modified strain produced up to 3.9 times more RMLs than the WT, yielding a maximum concentration of 14.62 g/L ...[more]

PMID: 28837648

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