Ontology highlight
ABSTRACT:
SUBMITTER: Luo C
PROVIDER: S-EPMC5570439 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Luo Chongyuan C Keown Christopher L CL Kurihara Laurie L Zhou Jingtian J He Yupeng Y Li Junhao J Castanon Rosa R Lucero Jacinta J Nery Joseph R JR Sandoval Justin P JP Bui Brian B Sejnowski Terrence J TJ Harkins Timothy T TT Mukamel Eran A EA Behrens M Margarita MM Ecker Joseph R JR
Science (New York, N.Y.) 20170801 6351
The mammalian brain contains diverse neuronal types, yet we lack single-cell epigenomic assays that are able to identify and characterize them. DNA methylation is a stable epigenetic mark that distinguishes cell types and marks regulatory elements. We generated >6000 methylomes from single neuronal nuclei and used them to identify 16 mouse and 21 human neuronal subpopulations in the frontal cortex. CG and non-CG methylation exhibited cell type-specific distributions, and we identified regulatory ...[more]