Dataset Information

Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry.

ABSTRACT: Claudin-1 is a hepatitis C virus (HCV) coreceptor required for viral entry. Although extensive studies have focused on claudin-1 as an anti-HCV target, little is known about how the level of claudin-1 at the cell surface is regulated by host vesicular transport. Here, we identified an interaction between claudin-1 and Sec24C, a cargo-sorting component of the coat protein complex II (COPII) vesicular transport system. By interacting with Sec24C through its C-terminal YV, claudin-1 is transported from the endoplasmic reticulum (ER) and is eventually targeted to the cell surface. Blocking COPII transport inhibits HCV entry by reducing the level of claudin-1 at the cell surface. These findings provide mechanistic insight into the role of COPII vesicular transport in HCV entry.IMPORTANCE Tight junction protein claudin-1 is one of the cellular receptors for hepatitis C virus, which infects 185 million people globally. Its cellular distribution plays important role in HCV entry; however, it is unclear how the localization of claudin-1 to the cell surface is controlled by host transport pathways. In this paper, we not only identified Sec24C as a key host factor for HCV entry but also uncovered a novel mechanism by which the COPII machinery transports claudin-1 to the cell surface. This mechanism might be extended to other claudins that contain a C-terminal YV or V motif.

SUBMITTER: Yin P

PROVIDER: S-EPMC5571274 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry.

Yin Peiqi P Li Ye Y Zhang Leiliang L

Journal of virology 20170824 18

Claudin-1 is a hepatitis C virus (HCV) coreceptor required for viral entry. Although extensive studies have focused on claudin-1 as an anti-HCV target, little is known about how the level of claudin-1 at the cell surface is regulated by host vesicular transport. Here, we identified an interaction between claudin-1 and Sec24C, a cargo-sorting component of the coat protein complex II (COPII) vesicular transport system. By interacting with Sec24C through its C-terminal YV, claudin-1 is transported ...[more]

PMID: 28679754

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Dataset Information

Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry.

Publications

Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry.

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