Unknown

Dataset Information

0

A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect.


ABSTRACT: The successful translation of cytotoxic T lymphocyte antigen-4 (CTLA-4) blockade has revolutionized the concept of cancer immunotherapy. Although monoclonal antibody therapeutics remain the mainstream in clinical practice, aptamers are synthetic oligonucleotides that encompass antibody-mimicking functions. Here, we report a novel high-affinity CTLA-4-antagonizing DNA aptamer (dissociation constant, 11.84 nM), aptCTLA-4, which was identified by cell-based SELEX and high-throughput sequencing. aptCTLA-4 is relatively stable in serum, promotes lymphocyte proliferation, and inhibits tumor growth in cell and animal models. Our study demonstrates the developmental pipeline of a functional CTLA-4-targeting aptamer and suggests a translational potential for aptCTLA-4.

SUBMITTER: Huang BT 

PROVIDER: S-EPMC5573796 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7195489 | biostudies-literature
| S-EPMC10497774 | biostudies-literature
| S-EPMC11344484 | biostudies-literature
| S-EPMC4840836 | biostudies-other
| S-EPMC10931446 | biostudies-literature
| S-EPMC6857638 | biostudies-literature
| S-EPMC7352693 | biostudies-literature
| S-EPMC3461908 | biostudies-literature
| S-EPMC3871234 | biostudies-literature
| S-EPMC9985596 | biostudies-literature