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Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis).


ABSTRACT: Tea plant is a typical fluorine (F) accumulator. F concentration in mature tea leaves is several hundred times higher than that in normal field crops. Long-term consumption of teas with high level F will increase the risks of dental and skeletal fluorosis. The mechanism of F accumulation in tea stands unclear. RNA-Seq and digital gene expression (DGE) techniques were used to investigate the effect of F on the differential expressions of transcriptome in tea plant. The results showed that F content in mature tea leaves was increased with increase in F concentration of cultural solution and duration of F treatment time. Based on comparison with data of GO, COG, KEGG and Nr databases, 144 differentially expressed unigenes with definite annotation were identified. Real-time reverse transcription PCR (qRT-PCR) was used to validate the effect of F on expression of 5 unigenes screened from the 144 unigenes. F treatment induced the expression of defense genes such as receptor-like kinases (RLKs) and U-box domain-containing protein. Based on the present study, F uptake is considered to be related to calcium-transporting ATPase, especially autoinhibited Ca2+ ATPase (ACAs) which was activated by the RLKs and worked as a carrier in uptake of F by tea plant.

SUBMITTER: Li QS 

PROVIDER: S-EPMC5575122 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis).

Li Qing-Sheng QS   Lin Xiao-Ming XM   Qiao Ru-Ying RY   Zheng Xin-Qiang XQ   Lu Jian-Liang JL   Ye Jian-Hui JH   Liang Yue-Rong YR  

Scientific reports 20170829 1


Tea plant is a typical fluorine (F) accumulator. F concentration in mature tea leaves is several hundred times higher than that in normal field crops. Long-term consumption of teas with high level F will increase the risks of dental and skeletal fluorosis. The mechanism of F accumulation in tea stands unclear. RNA-Seq and digital gene expression (DGE) techniques were used to investigate the effect of F on the differential expressions of transcriptome in tea plant. The results showed that F conte  ...[more]

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