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Patterning of sharp cellular interfaces with a reconfigurable elastic substrate.


ABSTRACT: Micropatterned cocultures are a useful experimental tool for the study of cell-cell interactions. Patterning methods often rely on sequential seeding of different cell types or removal of a barrier separating two populations, but it is difficult to pattern sharp interfaces between pure populations with low cross-contamination when using these approaches. Patterning by the use of reconfigurable substrates can overcome these limitations, but such methods can be costly and challenging to employ in a typical biology laboratory. Here, we describe a low-cost and simple-to-use reconfigurable substrate comprised of a transparent elastic material that is partially cut to form a slit that opens when the device is stretched. The slit seals back up when released, allowing two initially separate, adherent cell populations to be brought together to form a contact interface. Fluorescent imaging of patterned cocultures demonstrates the early establishment of a sharp cellular interface. As a proof of principle, we demonstrate the use of this device to study competition at the interface of two stem cell populations.

SUBMITTER: Curtis A 

PROVIDER: S-EPMC5575999 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Patterning of sharp cellular interfaces with a reconfigurable elastic substrate.

Curtis Allison A   Li David J DJ   DeVeale Brian B   Onishi Kento K   Kim Monica Y MY   Blelloch Robert R   Laird Diana J DJ   Hui Elliot E EE  

Integrative biology : quantitative biosciences from nano to macro 20170101 1


Micropatterned cocultures are a useful experimental tool for the study of cell-cell interactions. Patterning methods often rely on sequential seeding of different cell types or removal of a barrier separating two populations, but it is difficult to pattern sharp interfaces between pure populations with low cross-contamination when using these approaches. Patterning by the use of reconfigurable substrates can overcome these limitations, but such methods can be costly and challenging to employ in  ...[more]

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