Effect of Amino Acid Substitutions Within the V3 Region of HIV-1 CRF01_AE on Interaction with CCR5-Coreceptor.
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ABSTRACT:
SUBMITTER: Hongjaisee S
PROVIDER: S-EPMC5576193 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Publications
Effect of Amino Acid Substitutions Within the V3 Region of HIV-1 CRF01_AE on Interaction with CCR5-Coreceptor.
Hongjaisee Sayamon S Braibant Martine M Barin Francis F Ngo-Giang-Huong Nicole N Sirirungsi Wasna W Samleerat Tanawan T
AIDS research and human retroviruses 20170612 9
Specific amino acids within the V3 loop of HIV-1 CRF01_AE envelope glycoprotein that are involved in the interaction with CCR5/CXCR4 coreceptors, are not well characterized. We generated V3 mutants using polymerase chain reaction (PCR)-based site-directed mutagenesis of HIV-1 CRF01_AE R5-env plasmids at specific positions. Mutant viruses were produced by env-pseudotyped virus assay, tested for coreceptor usage using U373.R5 and U373.X4 cells, and viral entry was assessed with luciferase activity ...[more]
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