Unknown

Dataset Information

0

Imidacloprid Promotes High Fat Diet-Induced Adiposity in Female C57BL/6J Mice and Enhances Adipogenesis in 3T3-L1 Adipocytes via the AMPK?-Mediated Pathway.


ABSTRACT: Imidacloprid, a neonicotinoid insecticide, was previously reported to enhance adipogenesis and resulted in insulin resistance in cell culture models. It was also reported to promote high fat diet-induced obesity and insulin resistance in male C57BL/6J mice. Thus, the goal of the present study was to determine the effects of imidacloprid and dietary fat interaction on the development of adiposity and insulin resistance in female C57BL/6J mice. Mice were fed with a low (4% w/w) or high fat (20% w/w) diet containing imidacloprid (0.06, 0.6, or 6 mg/kg bw/day) for 12 weeks. Mice fed with imidacloprid (0.6 mg/kg bw/day) significantly enhanced high fat diet-induced weight gain and adiposity. Treatment with imidacloprid significantly increased serum insulin levels with high fat diet without effects on other markers of glucose homeostasis. AMPK? activation was significantly inhibited by 0.6 and 6 mg imidacloprid/kg bw/day in white adipose tissue. Moreover, AMPK? activation with 5-aminoimidazole-4-carboxamide ribonucleotide abolished the effects of imidacloprid (10 ?M) on enhanced adipogenesis in 3T3-L1 adipocytes. N-Acetyl cysteine also partially reversed the effects of imidacloprid on reduced phosphorylation of protein kinase B (AKT) in C2C12 myotubes. These results indicate that imidacloprid may potentiate high fat diet-induced adiposity in female C57BL/6J mice and enhance adipogenesis in 3T3-L1 adipocytes via the AMPK?-mediated pathway. Imidacloprid might also influence glucose homeostasis partially by inducing cellular oxidative stress in C2C12 myotubes.

SUBMITTER: Sun Q 

PROVIDER: S-EPMC5576855 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Imidacloprid Promotes High Fat Diet-Induced Adiposity in Female C57BL/6J Mice and Enhances Adipogenesis in 3T3-L1 Adipocytes via the AMPKα-Mediated Pathway.

Sun Quancai Q   Qi Weipeng W   Xiao Xiao X   Yang Szu-Hao SH   Kim Daeyoung D   Yoon Kyong Sup KS   Clark John M JM   Park Yeonhwa Y  

Journal of agricultural and food chemistry 20170727 31


Imidacloprid, a neonicotinoid insecticide, was previously reported to enhance adipogenesis and resulted in insulin resistance in cell culture models. It was also reported to promote high fat diet-induced obesity and insulin resistance in male C57BL/6J mice. Thus, the goal of the present study was to determine the effects of imidacloprid and dietary fat interaction on the development of adiposity and insulin resistance in female C57BL/6J mice. Mice were fed with a low (4% w/w) or high fat (20% w/  ...[more]

Similar Datasets

| S-EPMC6278525 | biostudies-literature
| S-EPMC6169790 | biostudies-literature
| S-EPMC6657571 | biostudies-literature
| S-EPMC5133865 | biostudies-literature
| S-EPMC5325319 | biostudies-literature
| S-EPMC3724828 | biostudies-literature
| S-EPMC6804195 | biostudies-literature
| S-EPMC11193219 | biostudies-literature
| S-EPMC3806839 | biostudies-literature
| S-EPMC4568043 | biostudies-literature