Unknown

Dataset Information

0

RECQL4 Promotes DNA End Resection in Repair of DNA Double-Strand Breaks.


ABSTRACT: The RecQ helicase RECQL4, mutated in Rothmund-Thomson syndrome, regulates genome stability, aging, and cancer. Here, we identify a crucial role for RECQL4 in DNA end resection, which is the initial and an essential step of homologous recombination (HR)-dependent DNA double-strand break repair (DSBR). Depletion of RECQL4 severely reduces HR-mediated repair and 5' end resection in vivo. RECQL4 physically interacts with MRE11-RAD50-NBS1 (MRN), which senses DSBs and initiates DNA end resection with CtIP. The MRE11 exonuclease regulates the retention of RECQL4 at laser-induced DSBs. RECQL4 also directly interacts with CtIP via its N-terminal domain and promotes CtIP recruitment to the MRN complex at DSBs. Moreover, inactivation of RECQL4's helicase activity impairs DNA end processing and HR-dependent DSBR without affecting its interaction with MRE11 and CtIP, suggesting an important role for RECQL4's unwinding activity in the process. Thus, we report that RECQL4 is an important participant in HR-dependent DSBR.

SUBMITTER: Lu H 

PROVIDER: S-EPMC5576896 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

RECQL4 Promotes DNA End Resection in Repair of DNA Double-Strand Breaks.

Lu Huiming H   Shamanna Raghavendra A RA   Keijzers Guido G   Anand Roopesh R   Rasmussen Lene Juel LJ   Cejka Petr P   Croteau Deborah L DL   Bohr Vilhelm A VA  

Cell reports 20160616 1


The RecQ helicase RECQL4, mutated in Rothmund-Thomson syndrome, regulates genome stability, aging, and cancer. Here, we identify a crucial role for RECQL4 in DNA end resection, which is the initial and an essential step of homologous recombination (HR)-dependent DNA double-strand break repair (DSBR). Depletion of RECQL4 severely reduces HR-mediated repair and 5' end resection in vivo. RECQL4 physically interacts with MRE11-RAD50-NBS1 (MRN), which senses DSBs and initiates DNA end resection with  ...[more]

Similar Datasets

| S-EPMC9156784 | biostudies-literature
| S-EPMC7935965 | biostudies-literature
| S-EPMC7162537 | biostudies-literature
2022-05-16 | GSE186085 | GEO
2022-05-16 | GSE186087 | GEO
| S-EPMC9640623 | biostudies-literature
2023-03-11 | PXD031357 | Pride
| S-EPMC9122494 | biostudies-literature
| S-EPMC6036208 | biostudies-literature
2022-05-16 | GSE186086 | GEO