Ontology highlight
ABSTRACT:
SUBMITTER: Meccariello A
PROVIDER: S-EPMC5577161 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Meccariello Angela A Monti Simona Maria SM Romanelli Alessandra A Colonna Rita R Primo Pasquale P Inghilterra Maria Grazia MG Del Corsano Giuseppe G Ramaglia Antonio A Iazzetti Giovanni G Chiarore Antonia A Patti Francesco F Heinze Svenia D SD Salvemini Marco M Lindsay Helen H Chiavacci Elena E Burger Alexa A Robinson Mark D MD Mosimann Christian C Bopp Daniel D Saccone Giuseppe G
Scientific reports 20170830 1
The Mediterranean fruitfly Ceratitis capitata (medfly) is an invasive agricultural pest of high economic impact and has become an emerging model for developing new genetic control strategies as an alternative to insecticides. Here, we report the successful adaptation of CRISPR-Cas9-based gene disruption in the medfly by injecting in vitro pre-assembled, solubilized Cas9 ribonucleoprotein complexes (RNPs) loaded with gene-specific single guide RNAs (sgRNA) into early embryos. When targeting the e ...[more]