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High-performance liquid chromatography purification of chemically modified RNA aptamers.

ABSTRACT: 2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many known RNA aptamers of therapeutic values, our protocol should be generally useful.

SUBMITTER: Lin CY

PROVIDER: S-EPMC5577503 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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High-performance liquid chromatography purification of chemically modified RNA aptamers.

Lin Chi-Yen CY Huang Zhen Z Jaremko William W Niu Li L

Analytical biochemistry 20131225

2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many kno ...[more]

PMID: 24373999

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