Ontology highlight
ABSTRACT:
SUBMITTER: Lin CY
PROVIDER: S-EPMC5577503 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Lin Chi-Yen CY Huang Zhen Z Jaremko William W Niu Li L
Analytical biochemistry 20131225
2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many kno ...[more]