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Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research.

ABSTRACT: Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.

SUBMITTER: Kim MS

PROVIDER: S-EPMC5582299 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research.

Kim Moon-Soo MS Kini Anu Ganesh AG

Molecules and cells 20170823 8

Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescrib ...[more]

PMID: 28835021

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