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ABSTRACT:
SUBMITTER: Zinke M
PROVIDER: S-EPMC5582604 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Zinke Maximilian M Fricke Pascal P Samson Camille C Hwang Songhwan S Wall Joseph S JS Lange Sascha S Zinn-Justin Sophie S Lange Adam A
Angewandte Chemie (International ed. in English) 20170707 32
Obtaining unambiguous resonance assignments remains a major bottleneck in solid-state NMR studies of protein structure and dynamics. Particularly for supramolecular assemblies with large subunits (>150 residues), the analysis of crowded spectral data presents a challenge, even if three-dimensional (3D) spectra are used. Here, we present a proton-detected 4D solid-state NMR assignment procedure that is tailored for large assemblies. The key to recording 4D spectra with three indirect carbon or ni ...[more]